Nestin Antibody AF2736 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemNestin Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF2736
ApplicationsWestern Blot, Immunohistochemistry, Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityRat
IsotypeIgG
HostGoat
TargetNestin
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
NCBI Full Gene Namenestin
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant rat Nestin, Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys), Accession # EDM00749
PurityImmunogen affinity purified
Brand/TrademarkR&D Systems, a Bio-Techne Brand
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Retinal gliosis and phenotypic diversity of intermediate filament induction and remodeling upon acoustic blast overpressure (ABO) exposure to the rat eyeExperimental Eye ResearchSeptember 1, 2023Lara A. Skelton, Sriganesh Ramachandra Ramachandra Rao, Rachael S. Allen, Cara T. Motz, Machelle T. Pardue, Steven J. FlieslerIHCb GFAP,Z0334,DAKO,Rabbit polyclonal,1:500 IHC Desmin,GTX103557,Genetex,Rabbit polyclonal,1:200 IHC1:1000 WB Nestin,AF2736,R&D,Systems Goat polyclonal,1:250 IHC Nestin,401/556309,BD Pharmingen,Mouse monoclonal,1:100 IHC1:1000

(2) Antiproliferative effect of boldine on neural progenitor cells and on glioblastoma cellsFrontiers in neuroscienceJanuary 1, 2023Jiménez-Madrona E, Morado-Díaz CJ, Talaverón R, Tabernero A, Pastor AM, Sáez JC, Matarredona ER.temperature in a solution containing the primary antibodies (goat anti-nestin antibody, Novus Biologicals AF2736, 1:100, to identify neural stem cells, together with rabbit anti-Cx43 antibody, Thermo Scientific 71–0700,

(3) Neonatal hyperoxia in mice triggers long-term cognitive deficits via impairments in cerebrovascular function and neurogenesisJournal of Clinical InvestigationNovember 15, 2022Marissa A. Lithopoulos, Xavier Toussay, Shumei Zhong, Liqun Xu, Shamimunisa B. Mustafa, Julie Ouellette, Moises Freitas-Andrade, Cesar C. Comin, Hayam A. Bassam, Adam N. Baker, et al.solution (1× PBS with 0.5% Triton X-100) with antibodies against nestin (1:500; R&D Systems, catalog AF2736), Sox2 (1:500; Abcam, catalog ab97959), and CD31 (1:200) overnight at room temperature with gentle shaking.

(4) The Rb/E2F axis is a key regulator of the molecular signatures instructing the quiescent and activated adult neural stem cell stateCell reportsNovember 1, 2022Fong BC, Chakroun I, Iqbal MA, Paul S, Bastasic J, O'Neil D, Yakubovich E, Bejjani AT, Ahmadi N, Carter A, et al.

(5) Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in miceNature communicationsSeptember 28, 2022O'Shea TM, Ao Y, Wang S, Wollenberg AL, Kim JH, Ramos Espinoza RA, Czechanski A, Reinholdt LG, Deming TJ, Sofroniew MV.Anaspec, AS-55043A); goat anti-Pdgfr-α (1:500, R&D systems, AF1062); goat anti-Nestin (1:500, R&D, AF2736); goat anti-Oct4 (1:500, R&D systems, AF1759); goat anti-Sox9 (1:500, R&D systems, AF3075); rabbit Aldh1l1

(6) Nestin contributes to laser choroidal and retinal neovascularizationMolecular visionJanuary 1, 2022Miloudi S, Valensi M, El Sanharawi M, Abitbol MM, Behar-Cohen F, Versaux-Botteri C.containing 5% skim milk, and then incubated overnight with a goat polyclonal nestin antibody (ref. AF2736, Bio-Techne, Lille, France) diluted at 1:500 and with a rabbit β-actin antibody diluted at 1:7,500 (ref.

(7) YAP/TAZ maintain the proliferative capacity and structural organization of radial glial cells during brain developmentDevelopmental biologyDecember 1, 2021Lavado A, Gangwar R, Paré J, Wan S, Fan Y, Cao X.(Vector laboratories #VP-RM04, 1:500), ZO1–Alexa-488 (Thermo #339188, 1:100), Nestin (R&D systems #AF2736, 1:100), ARL13b (ProteinTech #17711-1-AP, 1:250), β-Catenin–Alexa-647 (Cell Signaling #4627, 1:100),

(8) Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cellsThe Journal of biological chemistryNovember 1, 2021Chen L, Zhang M, Fang L, Yang X, Cao N, Xu L, Shi L, Cao Y.(Cell Signaling Technology, #5030), MYOD1 (Novus Biologicals, #NB100-56511), NESTIN (R&D systems, #AF2736), NEUROD1 (Cell Signaling Technology, #4373), NF-L (Cell Signaling Technology, #2837), PAX6 (Abcam,

(9) Microenvironment-responsive immunoregulatory electrospun fibers for promoting nerve function recoveryNature communicationsSeptember 9, 2020Xi K, Gu Y, Tang J, Chen H, Xu Y, Wu L, Cai F, Deng L, Yang H, Shi Q, et al.staining, rabbit anti-GAP-43 (Abcam, ab75810, 1:800) for axon staining, goat anti-nestin (R&D Systems, AF2736, 1:1000) for neural progenitor cell staining, mouse anti-GFAP (Service bio, GB12096, 1:800) for staining

(10) Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With AgingFrontiers in aging neuroscienceJanuary 1, 2020Sugiura A, Shimizu T, Kameyama T, Maruo T, Kedashiro S, Miyata M, Mizutani K, Takai Y.Ab was obtained as follows: Sox2 (sc-17320, Y17, Santa Cruz Biotechnology, Dallas, TX, USA), Nestin (AF2736, R&D Systems, Minneapolis, NE, USA), NeuN (MAB377X, Merck Millipore), glial fibrillary acidic protein

(11) Conditional Inactivation of Pen-2 in the Developing Neocortex Leads to Rapid Switch of Apical Progenitors to Basal ProgenitorsThe Journal of neuroscience : the official journal of the Society for NeuroscienceMarch 20, 2019Cheng S, Liu T, Hu Y, Xia Y, Hou J, Huang C, Zou X, Liang J, Stone Shi Y, Zheng Y, et al.raised against BrdU (ab6326; Abcam), Cleaved caspase-3 (CC3, #9661; Cell Signaling Technology), Nestin (AF2736; R&D Systems), Pax6 (PRB-278P; Covance), phospho-H3 (#9701; Cell Signaling Technology), Tbr2 (ab23345;

(12) Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortexIBRO reportsDecember 1, 2018Takamori Y, Hirahara Y, Wakabayashi T, Mori T, Koike T, Kataoka Y, Tamura Y, Kurebayashi S, Kurokawa K, Yamada H.IgG1,1/100,oligodendrocyte (immature) Nestin,"Rat nestin, 544-776 aa.","R&D systems Inc, Minneapolis, MN, USA,AF2736",Goat polyclonal,1/200,blood cell NeuN,"Purified cell nuclei from mouse brain (Mullen et al., 1992).

(13) Injectable polypeptide hydrogels via methionine modification for neural stem cell deliveryBiomaterialsSeptember 1, 2018Wollenberg AL, O'Shea TM, Kim JH, Czechanski A, Reinholdt LG, Sofroniew MV, Deming TJ.goat anti-mouse SOX9 (Novus, H00006662-M02,1:200 dilution), (vii) goat anti-mouse NESTIN (R&D Systems, AF2736, 1:800 dilution), (viii) rabbit anti-mouse ALDH1L1 (Abcam, ab87117, 1:500), (ix) rabbit anti-mouse S100β

(14) Nestin-positive microglia in adult rat cerebral cortexBrain researchMay 13, 2009Takamori Y, Mori T, Wakabayashi T, Nagasaka Y, Matsuzaki T, Yamada H.

(15) Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesisThe Journal of neuroscience : the official journal of the Society for NeuroscienceFebruary 11, 2009Gregorian C, Nakashima J, Le Belle J, Ohab J, Kim R, Liu A, Smith KB, Groszer M, Garcia AD, Sofroniew MV, et al.Cell Signaling 9552); rat anti-GFAP (1:1000, ZYMED Laboratories, 18-0063); goat anti-Nestin (1:50; R&D Systems, AF2736); rabbit anti-Cre (1:3000; Covance PRB-106C); goat antidoublecortin (1:500; Santa Cruz, sc8066); mouse

(16) Mechanisms of murine lacrimal gland repair after experimentally induced inflammationInvestigative ophthalmology & visual scienceOctober 1, 2008Zoukhri D, Fix A, Alroy J, Kublin CL.

(17) Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linkingBiotechnology and bioengineeringAugust 15, 2007De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD.

(18) Vasopressin mediates mitogenic responses to adrenalectomy in the rat anterior pituitaryEndocrinologyJuly 1, 2007Subburaju S, Aguilera G.

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Nestin Antibody

Nestin Antibody

Nestin Antibody

Nestin Antibody

Nestin Antibody

Nestin Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Nestin Antibody from R&D Systems, a Bio-Techne Brand

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